ABSTRACT

The effect of baffles on algal-bacterial biofilm structure and composition of

Chapter 2

The effect of baffles on algal-bacterial biofilm structure and composition of

zooplankton in wastewater stabilization ponds

Abstract

Nitrifier biomass in the water column of wastewater stabilization ponds is low and this

reduces nitrification rates. For this study, baffles were installed as attachment surface for

nitrifiers. Four pilot scale wastewater stabilization ponds were constructed in Kampala,

Uganda; pond 1 was control while ponds 2, 3 and 4 had baffles of the same total surface

area but different configurations. The ponds were operated for two periods i.e. under an

(period 1) and an influent BOD

(period 2, achieved by covering facultative pond). The

objective of this study was to investigate biofilm biomass and, distribution and composition

of algae on the baffles. The diversity and biomass of zooplankton in the pond water column

was also investigated. Dry weight of biofilm was determined gravimetrically while the wet

weight of the algae in the biofilm were determined using the bio volume method. The

results showed that the algal-bacterial biofilms grew rapidly within 2-3 weeks and after

that, the thickness did not increase. The algal-bacterial biofilm dry weights at 5cm depth of

all ponds during period 1 were higher than those of period 2. This is probably due to the

contribution of influent algae and heterotrophic biomass. As the facultative pond during

period 1 was not covered, more algae entered the maturation ponds forming thick biofilms

on the baffles, the algae in the deeper layers died forming a layer of detritus material.