ABSTRACT
Different immunohistochemical methods utilize the ability of specific antibodies to localize and bind to corresponding antigens in the cells and tissues. The binding reaction is labeled with a substance that either absorbs or emits light and hence produces a color. Fluorescent compounds, when excited by ultraviolet light, emit light which is dependent upon the nature of the compound. For example, fluorescein isothiocyanate emits green light, while rhodamine produces red fluorescence.1
