ABSTRACT
Electrochemiluminescence (ECL) is an electrochemical technique
combining the electrochemical reaction with luminescent detection.
The electrochemical reaction can lead to the formation of an exited
species, which then emits an optical signal for the detection of
the analyte and study of the ECL mechanism. Since the discovery
of light emission of Grigard compounds in 1927 and luminol
in 1929 at different applied potentials, many ECL emitters have
been presented, which promotes the quick development of ECL
investigation. These emitters can be used as labels to couple
with different biorecognition events, leading to a very powerful
biosensing technique. This technique integrates the advantages
of high sensitivity of luminescent detection, high specificity of
biological recognition, and controllability of electrochemistry and
thus has extensively been applied in different fields such as
clinic diagnostics, food and environmental monitoring, and even
biowarfare agent detection. This chapter covers the development
of ECL biosensing techniques, including the principle of ECL from
different labels, the strategy of ECL biosensor design, and the
application of ECL detection.
