ABSTRACT
Since the invention of atomic force microscopy (AFM) in 1986
[1], a variety of biological samples have been visualized using this
technique. AFM is now recognized as a versatile and powerful
analytical tool that can be used in many areas of biology. In this
chapter, we briefly overview how AFM works in imaging and
which factors affect successful imaging of biological samples, which
include the substrate, the cantilever, the setup, and image processing
(Fig. 2.1). Experimental protocols for specimen preparation of
biological samples, including DNA, protein, and cultured cells, are
also provided.
