chapter  12
4 Pages

Molecular Hybridization

Hybridization techniques are based on the physicochemical properties of nucleic acids: (1) the complementarity of the constituent bases of DNA (A/T, G/C) and RNA (A/U, G/C); (2) the reversibility of the process of separation of two strands of a DNA molecule (dénaturation) and of re-association of the two strands (re-naturation). Dénaturation is the dissociation of DNA strands (at 100°C or at alkaline pH) due to rupture of the hydrogen bonds that hold them together. The re-naturation process (re-formation of hydrogen bonds between the strands) is known as hybridization.