chapter  16
4 Pages

Screening of a Library

In order to identify the clones of interest, it is essential first to replicate the library. The bacterial colonies or lysis plates are transferred to a nitrocellulose or nylon membrane (see Profile 2). Their DNA is denatured by an NaOH incubation, fixed covalently on the membrane, and hybridized with different types of labelled nucleic probes in order to detect positive clones responding to the hybridization.