ABSTRACT
The refined characterization of a gene is done through sequencing, that is, through knowledge of the number, nature, and order of the nucleotides that make up the gene. Sequencing reveals, for example, the location of different restriction sites of the gene so that it can be better manipulated. Also, computerized translation of the nucleotide sequence into a sequence of amino acids can be used to confirm or propose a function of the protein coded by the gene.
