ABSTRACT
Antibodies have proved to be invaluable reagents for the detection and quantitation of many types of substances both in vitro and in vivo and have, therefore, found wide application in both the research laboratory and the diagnostics industry. Many of the techniques used in these two situations are similar, and thus it is helpful to review them together. In a chapter of this length it is impossible to cover all the ways that MAbs have been used in these areas; thus the emphasis in this chapter is on applications which are particularly widely used and those which are likely to benefit most from the ability to design engineered or modified antibodies.
