ABSTRACT
Surface plasmon resonance (SPR) imaging can be used as a
multidimensional instrument to characterize surface (coordinates
x and y) evolution (time t). In parallel, these surfaces can be functionalized and organized as biochips, provided they fulfill
requirements specific to label free biomolecular sensing. Getting
precise information about the thermodynamics of the probes
and targets interactions also necessitates precise microfluidic
conditions as well as enhanced image and data processing. Several
experimental approaches, such as biomolecular interactions, whole
cells analysis, or on-chip chemical synthesis, are presented herein,
illustrating the diversity of bio-analytical strategies compatible
with SPR imaging. Depending on the information needed, different
experimental setups can be used by taking advantage of other
dimensions such as incidence angle (θ), wavelength (λ), and
polarization (P ). We summarize here our different configurations, and their recent performances are reviewed. To further enhance the
plasmonic biochip reader capabilities, it is anticipated that one will
have to move from homogeneous gold film to other types of support
such as gold nanodisks or micro/nanostructures.
