chapter  6
Transcriptional Networks in Mammalian Gene Regulation
Pages 8

Recent advances in molecular biology and microarray technology have opened the door for massively parallel analysis of transcriptional networks. The development of chromatin immunoprecipitation methods allows for the assessment of transcription factor to target binding in vivo, and powerful amplification methods combined with “pro­ moter” or “enhancer” arrays that represent thousands of potential cis-regulatory elements enable the assessment of target occupancy on a global scale. This so-called location analysis, when combined with expression profiling of cells or tissues deficient for the transcription factor of interest in an “orthogonal analysis”, can differentiate direct from indirect targets. Combining multiple such analyses for various transcription factors with computational tools enables us to begin the assembly of the complex regulatory networks that govern transcrip­ tion in mammalian cells.