ABSTRACT
Examples of current applications of fluorescence include DNA sequencing, clinical assays, studies of metabolic enzyme activity, identification of biomolecules, and genetic analysis by fluorescence in situ. hybridisation.[1].. Fluorescence. occurs. in. many. molecules,. but. it. is. most. widespread. among aromatic compounds. Typical fluorescent substances, also referred . to as fluorophores, include fluorescein and rhodamin B. They can be covalently.attached.to.another.molecule,.such.as.a.protein.or.nucleic.acid,.in.a. procedure called fluorescent labelling. The most common approach involves fluorescent labelling of antibodies, which are then used as molecularly specific probes for detection of a particular biomolecular target.
