Preparation of Amylose–Polymer Inclusion Complexes in Phosphorylase-Catalyzed Enzymatic Polymerization (“Vine-Twining Polymerization”)

Poly(Tetrahydrofuran) Inclusion ComplexFirst example of the method leading to an amylose-polymer inclusion complex was achieved by amylose-forming polymerization in the presence of poly(tetrahydrofuran) (PTHF) (Mn = 4000) as a guest polymer [25]. The phosphorylase-catalyzed enzymatic polymerization of Glc-1-P from Glc7 as the primer was performed in the presence of a telechelic PTHF with hydroxy end groups in sodium citrate buffer at ca. 40°C (Fig. 7.2a). The resulting product was isolated and characterized by means of XRD and 1H NMR measurements. The XRD pattern of the product (Fig. 7.3a) was completely different from that of amylose (Fig. 7.3b) and PTHF, and was similar to that of the

Figure 7.1 Image of “vine-twining polymerization.”