ABSTRACT
A reconstituted protein synthesis system, comprising a minimal set o f components for the production o f active proteins, is described. The system consists o f 32 purified soluble factors from Escherichia coU together with other biochemical components, and is designated as the PU RE (Protein synthesis Using Recombinant Elements) system. The PURE system can be conveniendy used for both the production o f functional target proteins and for investigation o f protein translation and maturation processes. The synthesis o f various proteins, including model proteins, single-chain antibodies, multiple-domain proteins, etc., is examined. Moreover, biochemical ap plications for protein folding, particularly chaperone-mediated processes are further discussed.
