ABSTRACT
Heaters and On-Chip Sensors ........................................................................... 1059 25.3.2 Time Domain PDMS-Glass Hybrid Micro-PCR with Off-Chip
Heaters and Off-Chip Sensors ........................................................................... 1059 25.3.3 Time Domain SU-8-Based Micro-PCR with On-Chip Heaters
and On-Chip Sensors .......................................................................................... 1060 25.3.4 Space Domain PDMS-Glass Hybrid Micro-PCR with On-Chip
Heaters and Off-Chip Sensors ........................................................................... 1062 25.4 Conclusion ........................................................................................................................ 1062 References ................................................................................................................................... 1063
• Step 3: extension-temperature in this step is dependent on the DNA polymerase used. A commonly used temperature for Taq polymerase is 72°C. At this temperature, DNA polymerase starts to synthesize new DNA strands starting from the places where forward and reverse primers are left over in 5′ to 3′ direction.
