ABSTRACT
Our knowledge of RNA structures has advanced immeasurably since
the elucidation of the first such structure, that of yeast tRNAPhe, by
X-ray crystallography. Recent advances in massively parallel cDNA
sequencing (RNA-seq) have allowed new insights in transcriptomes
and greatly expanded the RNA universe. X-ray crystallography
and nuclear magnetic resonance (NMR) cannot cope with the
avalanche of newly discovered RNA molecules. This chapter focuses
on powerful well-tested methods for the analysis of the secondary
and tertiary structures of RNA molecules both in vitro and in vivo.
