ABSTRACT

Introduction e present emphasis on the diversication of global energy sources, while reducing the consumption of fossil fuels, has placed renewed interest on hydrogen as an alternative fuel and its synthesis through microbe-mediated bioconversions. Microbes have evolved unique mechanisms for hydrogen generation, and some of these mechanisms are being explored for biotechnological applications including nitrogenase-mediated and fermentative hydrogen production [1]. Eorts to improve hydrogen production have been focused on pathway redirection, identication and engineering of oxygen-tolerant hydrogenases, improvement in hydrogen molar yields and development of ecient hydrogen separation techniques from bioreactor headspace [1]–[5].