ABSTRACT
Introduction High-performance liquid chromatography (HPLC) is a potentially powerful tool for quantitative and qualitative analysis of substances possessing similar chemical characteristics. While varying column types, column conditions, and eluate characteristics generally enable peak resolution su cient for fractionation and analysis of similar chemical constituents in samples, such manipulations are often unsuccessful for ureide mixtures containing both asparagine and citrulline. Ureides are cyclic or acyclic acyl derivatives of urea and play key role in nitrogen metabolism and cycling in certain species of higher plants. Qualitative and quantitative analysis of these two ureides is critical for studies assessing Urea Cycle activity, a metabolic cycle involving nitrogen cycling in organisms, and factors aecting cycle functionality.
