ABSTRACT
One of the most important advances in biology has been our capacity to sequence the DNA of organisms. However, long after the conclusion of human genome sequencing, there are still regions of the genome that are unworkable; that is, they are difcult to mount and remain incomplete. Answers may come from second-generation sequencing, which has produced large volumes of data, generating millions of short reads per run, a reality that was unimaginable with Sanger sequencing.
