ABSTRACT
Anatoly N. Reshetilov, Nadezhda V. Zyrina, and Lyudmila A. Zheleznaya
Initiation of specic synthesis by deoxyribonucleic acid (DNA) polymerases is known to require a template-a single-stranded DNA-and a primer, a short oligonucleotide with the free 3′-OH end complementary to a template chain segment. After E. coli DNA polymerase I had been isolated, partially puried preparations of this enzyme were found to be capable of synthesizing long deoxyadenylate-deoxythymidylate (dAT) copolymers without primers and template added. This synthesis (named de novo or nonprimed DNA synthesis) is given considerable attention by investigators at present. Detailed analysis of the effect has revealed that thermophilic DNA polymerases synthesize highmolecular DNA from deoxynucleotide triphosphate (dNTP) in the absence of any initiating nucleic acid. This chapter briey reviews the molecular biological bases of de novo DNA synthesis and presents data in a novel approach to its analysis based on semiconductor transducers. Application of nonprimed synthesis techniques and novel DNA synthesis/sequencing detection methods
CONTENTS
9.1 Introduction ................................................................................................ 243 9.2 Molecular Biological Bases of De Novo DNA Synthesis ....................... 244 9.3 Electrochemical Approach to the Registration of Molecular
Effects in DNA ........................................................................................... 248 9.3.1 Direct Electrochemical Registration of DNA Synthesis ........... 248 9.3.2 Application of Semiconductor Instruments for DNA
Sequencing ...................................................................................... 251 9.3.3 Use of Semiconductor pH-Sensitive Transducers for
Registration of Nonspecic DNA Synthesis ..............................256 9.4 Conclusion .................................................................................................. 257 Acknowledgments .............................................................................................. 257 References ............................................................................................................. 257
is a valuable approach relating molecular biology on the whole and primed/ non-primed DNA synthesis in particular to green synthesis of nanoparticles.
