Tissue Engineering: The Therapeutic Strategy of the Twenty-First Century

Tissue Formation .......................................................................................18 1.4.5 Nucleic Acid Delivery for Tissue Engineering .................................................... 19

1.4.5.1 Techniques for Gene Delivery ................................................................. 19 1.4.5.2 Major Barriers in Gene Delivery and Conventional Solutions ........... 20 1.4.5.3 High-Throughput Approach to Identify Novel Biodegradable

Materials for Gene Delivery .................................................................... 21 1.4.5.4 Sustained DNA Release from Polymeric Scaffolds

for Tissue Engineering .............................................................................22 1.4.5.5 Targeted Gene Delivery for In Vivo Applications ................................22 1.4.5.6 Antisense Oligonucleotides and siRNA Delivery ................................23