ABSTRACT
It is believed that accurate peak definitions can have a great influence on reliability of the results provided through different spectroscopic techniques. As a result, and with the aim of putting this shortcoming aside, a wide range of the most frequently seen peaks are tabulated in this chapter. This will enhance understanding of the major peak assignments to specific functional groups that are present in complex biological molecules. For example, peaks in the area of 900 cm−1 are attributed to phosphodiester groups in both Raman and FTIR studies. The same finding can be seen in the area of 990-940 cm−1 (peaks related to C-C and C-O). More important peaks, such as those on the area of 1060-1030 cm−1 (C-C and C-O, mainly due to proteins and carbohydrates), 2000-1080 cm−1 (C-C, C-O, and phosphate of nucleic acids and membrane phospholipids, and partially proteins), 1350-1200 cm−1 (amide III region), 1600-1480 cm−1 (amide II region), 1800-1600 cm−1 (amide I region), and 3100-2800 cm−1 (CH region) also show considerable similarities in both techniques.
