ABSTRACT

Methods ............................................................................... 79 3.2.2 Integration of Mutagenic Oligonucleotides into Phage

Display Vectors ...................................................................................80 3.2.2.1 ssDNA Method .................................................................... 81 3.2.2.2 Cassette Methods ................................................................. 82 3.2.2.3 PCR Methods ....................................................................... 82

3.3 Random Mutagenesis ......................................................................................84 3.3.1 In Vitro Chemical Mutagenesis ..........................................................85 3.3.2 E. coli Mutator Strains........................................................................85 3.3.3 Error-Prone PCR.................................................................................85