ABSTRACT

Most microscopes today make use of built-in lamps. A current is passed through the lamp filament and the filament starts to radiate light. As noted in Chapter 1, the filament serves as a non-homogeneous source of light for the microscope and covers a range of visible light wavelengths at various intensities. When imaging a sample in a microscope, even illumination of the background is critical. The optical set up should provide homogeneous light at the highest possible intensity.