ABSTRACT
Understanding of cellular and molecular interactions as well as gene expression patterns that lead to a tissue condition necessitates extraction of homogeneous subpopulations or even single cell for further analysis. Cell sorting using §ow cytometry and af nity-labeled magnetic beads require prior dissolution of intercellular adhesion to allow separation of subpopulations from heterogeneous cell suspension. However, these techniques limit the extraction of intact tissue mass that may be critical in some instances for visual observations. Microdissection procedure originally involved manual guidance of needle to scrape and recover target region from thin tissue lm that was highly tedious, inaccurate, and operator dependent.1 To overcome these disadvantages, laser capture and
39.1 Introduction to Laser Capture and Microdissection .............................................................687 39.2 Principles and Instrumentation .............................................................................................688
