ABSTRACT
A number of modalities are available for messenger Ribonucleic acid (mRNA) targeting, and of these, the “antisense” strategies have been the most widely applied. Chemical optimization lead to improved nucleic acid molecules, but selection of which mRNA(s), and, of equal importance, which sequences within those mRNAs to target remain important issues. Self-quenching reporter molecules (SQRM) were dissolved in hybridization buffer and added to an excess of the target mRNA. Fluorescence intensity increased compared with background when a SQRM probe hybridized with the mRNA. An ever-expanding knowledge of the biochemical and molecular pathogenesis of hematologic malignancies continues to suggest new therapeutic targets Effects on K562 cluster and BFU-E and colony forming unit-GM colonies were determined and correlated with the ability to downregulate the targeted mRNA. Chemical optimization will lead to improved nucleic acid molecules, but selection of which mRNA(s), and, of equal importance, which sequences within those mRNAs to target remain important issues.
