ABSTRACT
The color of the stain must be different from that of the signal.
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The stain must not be so intense as to mask the signal.
Stains can be classified either from the chemical or the histological point of view. From the histological point of view, stains are divided into two groups:
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Natural stains
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Artificial stains
1. Natural stains
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Natural stains are extracts of animal or vegetable origin.