ABSTRACT
Chemiluminescence (CL) today is widely used in molecular biology and serology to detect oxygenfree radicals and adenosine triphosphate.
CL is light emission as a result of a chemical reaction. This light is detected by a photomultiplier with a high dynamic range, which allows quantitative measurement in an extended linear range from zero to millions of counts. The biological systems so employed, however, may be influenced by various conditions, such as storage, protein content, temperature, pH value and concentration of the light-emitting substance like luminol or firefly luciferase.
