ABSTRACT
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Nature has designed its biocatalysts to perform best in an aqueous surrounding, neutral pH,
and temperatures below 508C. However, these conditions are often contrary to the requirements of the process engineer or chemist to optimize a reaction with respect to volumetric
productivity or an easy downstream processing when substrates and/or products are not
easily soluble in water. To overcome these bottlenecks addition of organic solvents is common
practice. This has been used by various authors over the years, but a breakthrough was
achieved during the second half of the 20th century [1-3].
