chapter  7
24 Pages

Cell-Culture Media

Prokaryotic cell-culture medium is composed of salts, buffers, vitamins, hormones, amino acids, sugars or other carbon sources for use as food, and other defined compounds. Each cell line requires its own particular formula for optimal growth, and is available sterilized and bottled, ready for use, or in packaged and powdered form, as illustrated by recombinant

E. coli

medium in Table 7.2.