ABSTRACT
In the absence of carotene and serum lipids, the bilirubin concentration in a solution can be
directly measured by spectrophotometry on the basis of specific absorption at a wavelength
of 453 nm. According to Beer’s law, absorbance (A) is the logarithm of the ratio between the
intensity of light transmitted (I8) through a solution containing an absorbing substance and the intensity of light transmitted (I) in the absence of the absorbing substance: A ¼ log(I8/I).
