ABSTRACT
This chapter describes the most commonly used procedures for molecular marker analysis. Prior to molecular markers, linkage of useful traits was measured frequently to morphological markers. Isozyme analysis was the first type of molecular analysis widely employed. The first molecular maps were performed with allozymes. Likewise, in the first quantitative trait loci mapping experiments with molecular markers, allozyme loci were demonstrated to be linked to yield, fruit and seed weight, leaf ratio, and stigma exsertion, and it was demonstrated possible to select for yield by selecting for allozyme markers. Restriction Fragment Length Polymorphism analysis is the most widely used form of molecular marker analysis. Perhaps the greatest obstacle to the greater use of Restriction Landmark Genome Scanning is the technical expertise required. High-molecular weight DNA, three complete digests, and two electrophoreses are required.
