ABSTRACT
The preceding chapters have described in some detail the principles, methods, and applications of PCR-based DNA fingerprinting techniques currently used in the average plant genetic laboratory. This final chapter aims at introducing recent developments concerning (1) the identification and detection of single-nucleotide polymorphisms (SNPs) as the prototypes of third-generation molecular markers and (2) DNA microarray (so-called chip) technology as a highly sophisticated, nongel approach of DNA and RNA profiling. The treatment of these topics is necessarily brief, given that a comprehensive discussion of the explosive developments in these fields would easily fill another book. For recent reviews on the potential of SNP, microarray and expression profiling technologies in various fields of plant research, see Bhattramakki and Rafalski,
Brumfield et al.,
Gibson,
Gupta et al.,
Lemieux,
Morin et al.,
and Rafalski.
