ABSTRACT
This chapter describes several methods currently used to prepare tissues and to visualize nucleic acids.
8.2.1.1 Buffered formalin
❑
Fixative
Formalin 37.5%
200 ml
Phosphate buffer
1000 ml
❑
Phosphate buffer
Solution A
400 ml
Solution B
700 ml
❑
Solution A: disodium phosphate 0.1 M
Disodium phosphate
35.817 g
Distilled water
1000 ml
❑
Solution B: monopotassium phosphate, 0.1 M
Monopotassium phosphate
13.609 g
Distilled water
1000 ml
➫
Classic fixation by buffered formalin is one of the most often used methods for anatomopathologic studies.
