ABSTRACT
A probe consists of a labeled nucleic acid (DNA or RNA) whose nucleotide sequence is complementary to the target nucleic acid. The nucleotide sequence of a probe is antisense matching the sense sequence of the target. This chapter discusses different types of probes such as double-stranded DNA, single-stranded DNA, synthetic DNA and RNA. It discusses the advantages and disadvantages of the probes. The criteria for selecting the type of probe to use is also discussed. The chapter reviews Polymerase chain reaction (PCR) amplification, PCR protocol, asymmetric PCR amplification, and protocol for asymmetric PCR amplification. It discusses In Vitro transcription, precipitation techniques and purification principles.
