ABSTRACT
Embedding in hydrophilic resin allows a number of tissue samples to be tested at the same time. In situ hybridization can be carried out under conditions similar to those used for immuno-cytochemistry. Treatment of tissue for post-embedding in situ is similar to that for immunocytochemistry. This chapter discusses the principles of embedding techniques, types and characteristics of acrylic resin and choice of resins. Principles and protocols for embedding in lowicryl K4M resin, LR white resin and unicryl resin are described. The chapter discusses ultramicrotomy, deproteinization, denaturation of nucleic acid targets, and principles of immunocytochemical visualization.
