ABSTRACT
This chapter demonstrates the potential uses of in situ hybridization at the level of the light microscope using the same techniques employed for electron microscopy. In situ hybridization on semithin sections can be considered as a preliminary step to in situ hybridization on ultrathin sections (the intermediate step between light microscopy and electron microscopy). Semithin sections may be cut before or after visualization of in situ hybridization, depending on the methods such as unembedded tissue technique and pre-embedding technique. The chapter discusses different types of hybrids such as stable hybrids, unstable hybrids and partial hybrids. Different types of markers are described. The chapter reviews visualization of hybrids, choice of autoradiography emulsion and protocols for different types of hybrids. It discusses semithin frozen sections, semithin resin sections, and post-hybridization treatments.
