ABSTRACT
The Human Genome Project has facilitated the sequencing of many species, with demand for revolutionary technologies that deliver fast, inexpensive, and accurate information on the rise. Several next-generation sequencing devices have been introduced to the marketplace following sizable awards by the National Human Genome Research Institute and joint ventures, mergers, and acquisitions of large corporations. An unprecedented contest, the Archon X PRIZE for Genomics, further spotlights interest in next-generation technologies. In this review, DNA polymerasedependent strategies of single-nucleotide addition (SNA) and cyclic reversible termination (CRT), along with the DNA ligase-dependent strategy of sequencing by ligation (SBL), are discussed to highlight recent advances and potential challenges in genome sequencing.
