ABSTRACT
Clark Analysis in Heterogeneous Receptor Systems In a similar manner to that outlined above for the Schild analysis, Eq. (13) can be extended ( 40) to account for the interaction of a full agonist with two receptor types for which an antagonist has different affinities. This model, shown below, should be used to analyze data obtained from experiments in which a single-curve design has been used:
logw[A]so = log 10 [A]~ + logw ( 1 + (B][(aKoR + Koo + (B] (1 + a)] ) ( 14)
[B](aKoo + KoR) + Koo KoR (1 +a) where [A]50, [A]~. K80, KoR. and a are as defined previously. Fitting experimentally derived values of [A]s0 as a function of [B] to Eq. (14) allows estimates of K80, KoR. a, and [A]~ to be made.
