Nutriproteomics Approach to Understanding Dementia-Relevant Brain Protein Changes in Response to Grape Seed Extract, a Dietary Antioxidant

Abstract..............................................................................................................459 26.1 Introduction..............................................................................................459 26.2 Application of Proteomics Technologies in the Identification of

Targets of Antioxidants in Normal and Diseased Mammalian Brain......461 26.2.1 Normal Young Adult Rat Brain ...............................................461 26.2.2 Transgenic Model of Dementia, the Tg2576 Mouse...............461 26.2.3 Rodent Brain Tissue Preparation for Two-Dimensional

Electrophoresis........................................................................461 26.2.4 Derivatization of Protein Carbonyls with

2,4-Dinitrophenylhydrazine ....................................................462