chapter  9
12 Pages

The Freezing, Thawing, and Transfer of Cattle Embryos

Embryo Freezing ...................................................................................................120 Preparing Embryos for Freezing ...............................................................120

Traditional Method of Freezing Embryos.............................................................122 Direct Transfer Method of Freezing Embryos......................................................124 Embryo Thawing ...................................................................................................125 Cryoprotectant Removal (Dilution).......................................................................126 Glycerol Removal ..................................................................................................126 Ethylene Glycol Removal (Direct Transfer System) ............................................126 Transfer of Embryos..............................................................................................127 Summary ................................................................................................................127 References..............................................................................................................128