chapter  16
14 Pages

Alternative Methods to Micromanipulation for Producing Transgenic Cattle

Alternative Methods to Micromanipulation ..........................................................206 Microprojectile Bombardment ..................................................................206 Electroporation of Sperm ..........................................................................207 Sperm-Mediated Transfer Using Lipofection and Restriction Enzyme-Mediated Transfer .......................................................................211 Production of Transgenic Sperm Using Lipofection and Restriction Enzyme-Mediated Integration (REMI) .....................................................212 Production of Transgenic Calves ..............................................................212

Summary ................................................................................................................217 References..............................................................................................................217