ABSTRACT
I. Percoll sedimentation and Ficoll-Paque centrifugation .......................30 II. Magnetic cell sorting with anti-CD15 beads..........................................31
III. Functional assessment................................................................................32 A. Measurement of phagocytic activity .................................................32 B. Microbicidal assay ................................................................................34 C. Nitroblue tetrazolium dye reduction test .........................................35 D. Superoxide anion (O
) generation ....................................................36 E. Hydrogen peroxide formation............................................................37
