chapter  11
10 Pages

Buffers and media

ACK lysing buffer...............................................................................................230 Hemolytic Gey’s solution ..................................................................................230 Dispase/DNAase solution ................................................................................231 1X Gey’s solution................................................................................................231 Tris-buffered ammonium chloride...................................................................231 Glucose-phosphate-buffered saline (G-PBS) ..................................................231 1.0

M

Phosphate buffer, pH 7.6........................................................................231 0.3

M

Phosphate buffer......................................................................................232 Borate-buffered saline (BBS) .............................................................................232 Borate-buffered saline (BBS) (for Biotin label)...............................................232 Percoll mix solution............................................................................................232 Türk solution .......................................................................................................232 DMEM medium ..................................................................................................233 Labeling buffer ....................................................................................................233 Dialyzing buffer ..................................................................................................233 Carbonate-bicarbonate buffer ...........................................................................233 5X EGTA buffer ...................................................................................................233 Calcium-EGTA buffers .......................................................................................234 Hanks’ balanced salt solution with calcium and magnesium ....................234 Veronal buffered saline ......................................................................................234 Blocking buffer ....................................................................................................235 Cacodylate buffer................................................................................................235 Staining solution .................................................................................................235 Destaining solution.............................................................................................235 0.1

M

Sodium acetate buffer .............................................................................235 Acetate/EDTA buffer .........................................................................................236 Borate buffers: pH 8.5, 9.0, and 10.0................................................................236 0.1

M

Diethanolamine buffer, pH 9.8 ..............................................................236 DTNB solution.....................................................................................................237 0.3% Glutaraldehyde..........................................................................................237

0.01