chapter  1
34 Pages

Cells

I. Preparation of cell suspensions................................................................2 A. Heat inactivation of fetal calf serum...............................................2 B. Spleen ...................................................................................................3 C. Bone marrow.......................................................................................4 D. Peritoneal cells ....................................................................................5 E. Blood collection ..................................................................................6 F. Cell counting .......................................................................................6 G. Cell counting using Türk solution ..................................................7 H. Determination of cell viability .........................................................7