chapter  2
18 Pages

Flow cytometry

I. Overview....................................................................................................35 A. Labeling of antibodies with fluorescein isothiocyanate ............38 B. Labeling of antibodies with biotin ................................................39 C. Alternate method for biotinylation of antibodies.......................39 D. Labeling antibodies with Texas red ..............................................40 E. Staining of cells for detection of surface antigens......................41 F. Staining of cells for detection of surface antigens

in plates..............................................................................................41 G. Detection of intracellular antigens ................................................42 H. Influx of Ca

ions using Fluo-3.....................................................44 I. Influx of Ca

ions using Indo-1 ....................................................45 J. The respiratory burst of neutrophils.............................................45 K. Alternative technique using DCHF-DA .......................................47 L. H

O

production measurement using dihydrorhodamine 123 ....................................................................47