Quantitative Morphology in Relation to Long-Term Pain States: Estimates of Neuron Numbers

Assumptions......................................................................218 11.3 Discussion of the Steps in a Stereologic Protocol for Estimating

Neuron Numbers ..........................................................................................218 11.3.1 Introduction ......................................................................................218 11.3.2 Serially Section the Tissue Containing the Reference Space .........219 11.3.3 Select the Sections for Sampling.....................................................219 11.3.4 Define the Reference Space.............................................................219 11.3.5 Count the Neurons ...........................................................................220