ABSTRACT

Agar ..................................................................20.0g N-Z-Amine A...................................................10.0g NaCl ....................................................................5.0g Yeast extract........................................................5.0g MgCl2·6H2 .......................................................2.0g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pressure-121°C. Allow tubes to cool in a slanted position.