ABSTRACT
Because many enzymes contain a histidine residue, which is critical for the catalytic process, the site-specific modification of this residue has been the subject of many studies. Most of these studies have been directed at the study of the catalytic mechanism of enzymes and a few at protein-protein interactions or substrate/cofactor binding. Thus, despite the importance of histidine, only a small number of reagents have been studied. Most of the current work reported in the literature is with diethylpyrocarbonate.
