ABSTRACT
The group-specific modification of arginine is relatively easy to achieve; it is somewhat more difficult to obtain the site-specific modification of arginine. Arginine residues are usually involved in binding sites rather than catalytic sites. Thus, modification of arginine rarely results in the total loss of activity. As a result, it is critical to take residual activity into consideration in determining the kinetics of inactivation. The author has found the approach developed by Levy and co-workers
quite useful. This approach has been used in a number of studies on the site-specific modification of proteins.
