ABSTRACT

Amyloidosis is a diverse group of diseases characterized by extra-cellular tissue deposits of abnormally folded proteins that are generically termed amyloid and that occur in one or many organs. Positive samples included tissues positive for amyloid by Congo red stain and deposits that were fibrillar by electron microscopy. Affinity reagents targeted against amyloid deposits were prepared by derivatizing a proprietary affinity ligand onto both reactive IDM Affinity Beads or RS100 Reactive ProteinChip Arrays via a free primary amine. The most frequent type of systemic amyloidosis is amyloid derived from the immunoglobulin light chain; however, other types of amyloid have to be considered in the differential diagnosis. Surface enhanced laser desorption/ionization-Mass Spectrometry is a technology that combines solid-phase extraction chromatography with direct detection by mass spectrometry.